C. elegans protein extraction.
R. John Lye
rjl6n at uva.pcmail.virginia.edu
Fri Dec 12 16:54:26 EST 1997
Stuart Rison wrote:
> He claims that, in his previous attempt, C. elegans worms survived polytron
> homogenisation and sonication in a triton X-100 buffer (i.e. there were
> still some worms intact and ALIVE post-treatment).
Yeah, they're tough little buggers.
> Is there a more succesful technique?
I used a French press set at 5,000 psi internal pressure.
e-mail to: rjl6n at Virginia.EDU
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