C. elegans protein extraction.

R. John Lye rjl6n at uva.pcmail.virginia.edu
Fri Dec 12 16:54:26 EST 1997


Stuart Rison wrote:
>  He claims that, in his previous attempt, C. elegans worms survived polytron
> homogenisation and sonication in a triton X-100 buffer (i.e. there were
> still some worms intact and ALIVE post-treatment).

Yeah, they're tough little buggers.

> Is there a more succesful technique?

I used a French press set at 5,000 psi internal pressure.

good luck,
-- 
John Lye

e-mail to: rjl6n at Virginia.EDU
homepage: http://avery.med.virginia.edu/~rjl6n/homepage.htm



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