Mnl I - non-palindromic rare-cutter?
Ian & Viive Howell
ihowell at ozemail.com.au
Sat Dec 13 04:33:47 EST 1997
The enzyme Mnl I is used for one of our assays to detect a G-A
mutation by the loss of a restriction site. The recognition sequence
for Mnl I is CCTC, so how could it detect a G-A mutation? The NEB
"REBASE" site gives the recognition sequence only as CCTC, but I have
finally found 1 textbook reference to Mnl I recognising CCTC & GAGG.
My question is - (other than by trial and error) how and where can you
find out whether an enzyme will cut additional sequences? Does
anybody know of other similarly acting enyzmes?
Laboratory & Community Genetics
Kolling Institute of Medical Research
Royal North Shore Hospital
NSW AUSTRALIA 2065
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