BclI

Richard L lm11 at gpu.srv.ualberta.ca
Mon Dec 15 15:13:20 EST 1997


Per Mygind <perm at medmicro.aau.dk> wrote:
: -- Has any of you ever got a problem, using the restrictionenzyme BclI,
: it does not seem to cut in my hands ???

: ************************************************************************
: If you are are not part of the solution, you are part of the precipitate
: ************************************************************************

: Per Mygind, Cand.scient

: Department of Medical Microbiology and Immunology
: The Bartholin Building
: University of Aarhus
: Denmark
: phone : 89 42 17 47
: fax   :

Hi there,

Have you check the reaction temp. BclI's optimal temp should be >50C, not
the usual 37C.
 
Richard



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