GST pull down vs. 6X his- which method is better?
bobl at u.washington.edu
Mon Dec 15 13:46:29 EST 1997
I wish to confirm a protein interaction identified in a yeast two-hybrid
screen using a yeast-independent, in vitro system. I am considering a GST
pull down assay or an approach using metal affinity chomatography with a
6X his fusion. The protein interaction I am working with is
moderate to weak in binding affinity. Which of these two methods is more
robust in detecting weak protein interactions? Are there better methods I
should consider? All responses will be gratefully accepted.
Bob Livingston voice: (206)543-2850
Dept of Pathology fax: (206)685-4829
University of Washington email: bobl at u.washington.edu
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