mammalian protein expression vector

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Tue Dec 16 10:38:13 EST 1997


Hi all,
I am looking for a mammalian expression vector with the following
characteristics:
* strong promotor (not necessarily inducible)
* C-terminal tag (myc, HA or alike preferred, but not 6xHis)
* possibly an SV40 replication origin for episomal propagation in cos cells

I have seen a vector by Boehringer Mannheim, pMH, that would be ideal for
my studies, but it is not yet available. 
Any ideas for a good vector? 

TIA
Frank



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