EDC cross-linking of proteins

Mike Mayer mimayer at indiana.edu
Tue Dec 16 11:31:34 EST 1997


Does anyone have some good, recent references for EDC (zero-length)
crosslinking of proteins? I am attempting to covalently cross-link a
dimer and don't know what protein or EDC concentrations to use, also,
how long should the reaction go, and how should I quench it?

Thanks a lot for any advice you have.

Krsiten MacLaughlin, PhD




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