method to anchor non-adherant cells for Calcium imaging?
jtsuruta at med.unc.edu
Tue Dec 16 17:46:18 EST 1997
Help! We are attempting to image calcium fluxes in spermatogonia after
loading them with FURA-2 and treating them with various growth factors.
The problem: spermatogonia are non-adherant cells and wash off of our
coverslips making subsequent manipulations problematic :(
We've tried/are trying to use coverslips coated with:
Collagen I or IV
laminin + collagen IV
These methods do not anchor our spermatogonia very tightly. Does anyone
have a protocol for anchoring non-adherant cells to coverslips or
Thanks in Advance,
Laboratories for Reproductive Biology
University of North Carolina at Chapel Hill
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