RNA Sequencing Help!!!

Richard L lm11 at gpu.srv.ualberta.ca
Tue Dec 16 21:17:06 EST 1997


ls10 at ZSU.EDU.CN wrote:
: Hello,

: I want to sequence a 600bp mRNA. Is there any commercial kit for direct
: RNA sequencing? Should I have to retrotranscribe it into cDNA first? Any
: practical protocol and suggestion is warmly welcomed. 

: Thanks!

: Black

Hi Black,

RNA sequencing is almost the same as the common DNA sequencing, what you
need is: 4 dNTPs and reverse transcriptase. of course you need to label
your primers.

Hope this helps

Richard



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