GFP and damage

ronlab ronlab at uic.edu
Tue Dec 16 16:58:05 EST 1997


Jillian Johnston wrote:
> 
> I am making trangenic Xenopus with an inserted GFP gene.  Is the
> wavelength that is used to view GFP damaging to the developing
> tadpoles? Thanks
> 
> --
> Jillian Johnston
> Dept. of Biological Sciences       Guider
> Univ. of Calgary                   30 Pathfinders, Tanisi District

Which form of GFP are you using? I would suggest EGFP (FITC - like) and 
short exposure times. Tissue culture cells tolerate it very well.

Eugene Kandel.
U09577 at uic.edu



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