Subtractive hybridization. What is "HAP"?

Hiranya Roychowdhury hroychow at NMSU.EDU
Thu Dec 18 10:53:00 EST 1997

At 02:32 PM 12/17/97 -0800, Jun-Ichi Aikawa wrote:
>Hi, I need your help.
>I saw comment attached to a clone of my interests which was found in
>Genbank EST database. What is "HAP"?
>"Equal amounts of plasmid DNA from three normalized libraries (melanocyte
>2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss
>circles were made in vitro. Following HAP purification, 


this DNA was used
>as tracer in a subtractive hybridization reaction. The driver was
>PCR-amplified cDNAs from pools of 5,000 clones made from the same 3
>libraries. The pools consisted of I.M.A.G.E. clones 260232-265223,
>340488-345479, and 484488-489479."
>Jun-ichi Aikawa, Ph.D.
>University of California, San Diego

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at

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