Self ligation of PCR products

Steven Kirov kirov at medfac.acad.bg
Fri Dec 19 10:21:59 EST 1997


Hi there,
Generally if your PCR primers don't have PO4 on their 5' end there is no 
way to get ligation (or at least this is what is accepted up to now - 
prove diopposite and get a Nobel prize?).
If they do have, than you should know, that part of the molecules have G, 
C or T as an extension instead of A (though a small percent). And also as 
 our colleague Hiranya Roychowdhury writes there are also blunt end ones.
Greetings to all and Merry Christmas and so on...

Steven Kirov
PhD student
Dept. of Biochemistry
Medical Faculty
Sofia
Bulgaria




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