Why pT7lac promoter is so leak

Thomas Tomzynski tomtomz at EARTHLINK.NET
Fri Dec 19 18:36:07 EST 1997


Dear People,

The addition of glucose to repress leaky lac promoters is often advised
here.  I assume this is because of catabolite repression.  Does it still
apply to lacUV5 promoters as in BL21[DE3]?  I thought lacUV5 was not
subject to catabolite repression.  Does the glucose make a measurable
difference for people out there?

Thanks,
Tom



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