How do you transform fibroblast cells for making cell lines?

David N. Levy levy at
Tue Dec 23 15:56:50 EST 1997

How can you take primary fibroblast cells and infect or transfect them
in order to transform them?  Can you transfect the ad. E1A gene, or
papillomavirus E7 or E6, or infect with SV40 or what?  Thanks.

David N. Levy
University of Alabama at Birmingham
Birmingham, AL 35294-0007

levy at

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