Ion Exchange Chromatography Question

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Sat Dec 27 21:34:40 EST 1997


In article <6824a6$env$1 at news01.iafrica.com>, "C Cantina" <ccantina at iafrica.com> wrote:
>Hi,
>
>I have the following problem: A crude plant extract should be fractionated
>using ion exchange chromatography and electrophoresis. However, I do at this
>point not know what constituents are to be expected. So I am looking for a
>chromatography procedure which will be most suitable in this case, e.g.
>should I uses a strongly acidic cation exchange?

It is purely empiric matter if you don't know the properties of the stuff. 
If a protein, in most cases weak anion exchanger is preferable.

- Dima



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