How large can be cloned into pUC19 ?

duncant at vax.cs.hscsyr.edu duncant at vax.cs.hscsyr.edu
Mon Dec 29 22:27:28 EST 1997


>..  I wonder the biggest size that can be cloned into pUC19 but not quite influence pUC19's high copy character in host(Ecoli). Thank you!     
> linchengtao
> 
> 
We have constructs in pUC119 (a pUC19 derivative) with an insert of approx. 7 Kb, and the plasmid still shows very high copy number. I'm sure it could tolerate somewhat larger.


                       cheers for a happy new year,

                       Tom Duncan
                       Research Ass't Prof of Biochem. & Mol. Biol.
                       SUNY Health Science Ctr
                       Syracuse, NY
              




More information about the Methods mailing list