Will Dpn I cut half-methylated DNA?
GR Taylor PhD MRCPath
G.R.Taylor at dial.pipex.com
Tue Dec 30 17:24:50 EST 1997
No, its failure to cut hemi-methylated hybrids is essential for the
technique of genome mismatch scanning.
Wolfgang Schechinger <wgschech at med.uni-tuebingen.de> wrote in article
<688nvp$j8j at mserv1.dl.ac.uk>...
> Salvete, all you lab-slaves condemned to work on
> holy holidays in the dungeons of molecular
> biology laboratories!
> I want to get rid of PCR-hybrids derived from plasmids produced in a
> dma+ host strain. Will DpnI do that job for me?
> Thanks for answering!
> This message is RNAse free - please don't touch!
> Wolfgang Schechinger
> University of Tuebingen
> email: wgschech at med.uni-tuebingen.de
> !Junk mail is *not* appreciated!
> SPAMs will be answered automatically by sending my 128MByte virtual
> This is *NO* joke.
> usual disclaimers apply
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