Will Dpn I cut half-methylated DNA?

GR Taylor PhD MRCPath G.R.Taylor at dial.pipex.com
Tue Dec 30 17:24:50 EST 1997


No,  its failure to cut hemi-methylated hybrids is essential for the
technique of genome mismatch scanning.

Graham Taylor

Wolfgang Schechinger <wgschech at med.uni-tuebingen.de> wrote in article
<688nvp$j8j at mserv1.dl.ac.uk>...
> Salvete, all you lab-slaves condemned to work on 
> holy holidays in the dungeons of molecular 
> biology laboratories!
> 
> I want to get rid of PCR-hybrids derived from plasmids produced in a 
> dma+ host strain. Will DpnI do that job for me?
> 
> Thanks for answering!
> 
> Wolfi
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