SYPRO Orange Quenching by 35-S

Dick Haugland dick at probes.com
Sat Feb 1 00:52:13 EST 1997


The SYPRO dyes, which were developed by Molecular Probes, bind to the
lipid coating around proteins in SDS gels and not to the protein itself.
I would not expect any "interaction" with 35-S.  If necessary, the dyes
can be extracted from the proteins with organic solvents such as alcohol
and, following transfer, do not interfere with immunoblotting.

Dick Haugland, President
Molecular Probes, Inc.
dick at probes.com



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