SYPRO Orange Quenching by 35-S

Dick Haugland dick at
Sat Feb 1 00:52:13 EST 1997

The SYPRO dyes, which were developed by Molecular Probes, bind to the
lipid coating around proteins in SDS gels and not to the protein itself.
I would not expect any "interaction" with 35-S.  If necessary, the dyes
can be extracted from the proteins with organic solvents such as alcohol
and, following transfer, do not interfere with immunoblotting.

Dick Haugland, President
Molecular Probes, Inc.
dick at

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