MMLV and rNTP's

Matt Thomas Thomas at molbio.uoregon.edu
Sat Feb 1 09:15:47 EST 1997


I have a question about MMLV.  I'm useing it to do some primer ext on
RNA generated in vitro by human RNA pol II.  I have a very strange
result that I could explain if the MMLV was useing rNTPs as substrates. 
The rNTPs are left over from the transcription reaction.  Their
concentarion could be anywhere from 1mM to 2.5mM.  My dNTPs in the
primer ext reaction are about 300µM.    

Does anyone know if this enzyme will use rNTPs on an RNA template?  

thanks,

Matt Thomas
Thomas at molbio.uoregon.edu



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