PCR product into northern probe ??
xie at made.biochem.mpg.de
Sat Feb 1 11:54:26 EST 1997
u8251012 at cc.kmc.edu.tw wrote:
: Dear netter:
: I have some question in my lab works
: I decided to make my RT-PCR product into a northern probe
: but RT-PCR product have only 123bp.
: What's the best Radio-labelling method with maximal efficiency and
You can use random priming method and add 1-2pmol your antisense primer
to the labelling mixture. This combination works well in my hand.
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