PCR product into northern probe ??

Youhua Xie xie at made.biochem.mpg.de
Sat Feb 1 11:54:26 EST 1997


u8251012 at cc.kmc.edu.tw wrote:
: Dear netter:
: I have some question in my lab works
: I decided to make my RT-PCR product into a northern probe
: but RT-PCR product have only 123bp.
: What's the best Radio-labelling method with maximal efficiency and 
: radio-intensity.?
You can use random priming method and add 1-2pmol your antisense primer 
to the labelling mixture. This combination works well in my hand.

xie




More information about the Methods mailing list