PCR product into northern probe ??

u8251012 at cc.kmc.edu.tw u8251012 at cc.kmc.edu.tw
Sat Feb 1 01:16:00 EST 1997


Dear netter:
I have some question in my lab works
I decided to make my RT-PCR product into a northern probe
but RT-PCR product have only 123bp.
What's the best Radio-labelling method with maximal efficiency and 
radio-intensity.?


=>primer extension ? radiolabeling by PCR primer itself for priming !

=>cocantemerization of RTPCR product 
    and followed by random priming labelling

=>end labelling by T4 kinase with 32P-gamma ATP as a substrate
  

THANKS

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