Boehringer DIG system experiences
a03m at biologie.uni-bremen.de
Sun Feb 2 12:37:41 EST 1997
kferri at email.unc.edu (Karen Ferri) wrote:
>I am currently trying Boehringer's DIG Labeling and Detection kit b/c I'd
>like for our lab to switch over (me, at least) to non-radioactive probing.
>The instructions call for positively charged nylon membranes. The one we
>have in lab is by Amersham and I imagine it is uncharged, since it didn't
>say anything on the box or instructions.
>I did my control labeled DNA filter and the spots are black. This was on
>the Amersham filter. SO is a positively charged filter truly needed? I'd
>like to find out others' experiences before I go through the trouble of
>preparing a membrane with my experimental DNA
we use Boehringer DIG since many years successfully with Amersham's
Hybond N+ (charged membrane). We also use uncharged membranes
from time to time, but as blotting is easier and faster with charged
membranes (simply alkali-blotting and no baking or crosslinking
required) and as one can be sure that no nucleic acids will go
through, we prefer N+.
(no affiliation bla bla...)
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