HOW to Eliminate PCR primer dimer
natalieB at qimr.edu.au
Sun Feb 2 23:55:13 EST 1997
educe primees dimers: add the PRIMERS last and ON ICE in the tubes;
reduce the quantity of primers;
the increase in annealing temp will only help reduce the
To obtain a stronger signal you could add 1 or 2 cycles,
or dilute the product obtained 1/100 and rerun another PCR
with only 25 cycles
: Dear netter:
: I have Some questions about PCR (polymerase chain rex)
: and need your advices !
: 1.How can I remove (or reduce) the primer dimer in PCR reaction
: how about increase annealing Temp ? or lowering Ma2+ conc.
: 2.After 38 cycles, a faint signal was obtained. Can I increase
: cycle number, or template DNA or even dNTP content
: TO further INCREASE THE SIGNAL I WANT ??
: 3.What's the best methods to elute out the PCR product with
: high yields and low DNase contaminated. ANY kits suggested ?
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