pH for RNA work

John Morlan jmorlan at SFSU.EDU
Mon Feb 3 21:49:17 EST 1997


Banwari,

We rinse our electrode in DEPC-treated water.  Strictly speaking, this does
not make the electrode RNAse free, but we seldom have any problems doing it
this way.

-John
SFSU


>Hi!
>While making RNAse free Tris, does the pH electrode have to be
>RNase free for adjusting the pH? If yes, then how do you make an
>electrode RNase free?
>Thanks in advance
>
>*******************************************************************
>* Banwari Lal, Ph.D.                                              *
>* Microbial Biotechnology,                                        *
>* TATA Energy Research Institute,                                 *
>* India Habitat Centre, Lodhi Road,                               *
>* New Delhi 110 003. INDIA.                                       *
>* Phone: 91-011-462 2246; 91-011-460 1550                         *
>* Fax: 91-011-462 1770; 91-011-463 2609                           *
>* Website: http//www.terin.org                                    *
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