Growth and transformation of XL1-Blue

Kevin A. Mulcahy K.Mulcahy at sheffield.ac.uk
Mon Feb 3 12:54:30 EST 1997


Hi,

I have XL1-blue cells and was wondering what the optimal conditions for 
growth and high-efficiency transformation? Can they be grown in LB 
medium containing 20mM magnesium sulphate and made competent using the 
rubidium chloride procedure described in Promega's "Protocols and 
Applications Guide"?

Many thanks,

Kevin Mulcahy.

ps: If you need me to write out the transformation procedure then let me 
know and I'll post it to the list.



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