roller bottle culture

shanthi adimoolam shanth at students.uiuc.edu
Mon Feb 3 12:24:07 EST 1997


Hello everyone,
I was wondering if anyone has had experience with roller bottle culture 
for scale up of mammalian cells. If so i was wondering if i could get any 
pointers to references or protocols. Basically, i was interested in 
information like minimum/maximum volume of media to be used/square cm of 
surface area, speed during seeding and during growth. Any tips would be 
helpful, since i am new to this.
Thanks in advance,
-shanthi
 



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