IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

PCR primer-dimer elimination

Robert B. Chadwick chadwirb at perkin-elmer.com
Mon Feb 3 11:08:53 EST 1997

The easiest method to eliminate (or at least greatly reduce) PCR
primer-dimer formation is to do a hot-start.  There are three ways to do

	1)  Don't add DNA Polymerase enzyme until the reactions reach
approximately 80C.

	2)  Separate the enzyme from the primers by using a wax bead overlay
which melts at a higher temperature. 

	3)  Use a hot start DNA Polymerase which is inactive until it reaches
elevated temperatures.

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net