PCR primer-dimer elimination

Robert B. Chadwick chadwirb at perkin-elmer.com
Mon Feb 3 11:08:53 EST 1997


The easiest method to eliminate (or at least greatly reduce) PCR
primer-dimer formation is to do a hot-start.  There are three ways to do
this:

	1)  Don't add DNA Polymerase enzyme until the reactions reach
approximately 80C.

	2)  Separate the enzyme from the primers by using a wax bead overlay
which melts at a higher temperature. 

	3)  Use a hot start DNA Polymerase which is inactive until it reaches
elevated temperatures.



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