Vol of ligation mix to use in transformation

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Mon Feb 3 10:41:09 EST 1997


In article <kwarner-0202971344000001 at cha-nc1-08.ix.netcom.com>,
kwarner at unccvm.uncc.edu (Keith Warner) wrote:


> There was a report in Biotechniques last month (?) which discussed using
> an agarose well prepared in a microfuge tube to clean DNA before
> ligation.  

<snip>

The article was "Simplified desalting of ligation reactions immediately
prior to electroporation into E. coli" by AM Atrazhev and JF Elliott -
Biotechniques v.21(6) pg. 1024, Dec. 1996.

Cheers

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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