Boehringer DIG system experiences
a8803349 at unet.univie.ac.at
Mon Feb 3 02:55:02 EST 1997
On 2 Feb 1997 03:30:48 GMT, kferri at email.unc.edu (Karen Ferri) wrote:
>I am currently trying Boehringer's DIG Labeling and Detection kit b/c I'd
>like for our lab to switch over (me, at least) to non-radioactive probing.
>The instructions call for positively charged nylon membranes. The one we
>have in lab is by Amersham and I imagine it is uncharged, since it didn't
>say anything on the box or instructions.
>I did my control labeled DNA filter and the spots are black. This was on
>the Amersham filter. SO is a positively charged filter truly needed? I'd
>like to find out others' experiences before I go through the trouble of
>preparing a membrane with my experimental DNA
I have tried the recommended Boe-Ma membrane(positively charged) and
the uncharged Nytran membrane (Schleicher&Schuell) and I get less
specific staining and more background using the Boe-Ma.
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