Boehringer DIG system experiences

[Martin Offterdinger]

On 2 Feb 1997 03:30:48 GMT, kferri at email.unc.edu (Karen Ferri) wrote:

>
>
>HI everyone!
>
>I am currently trying Boehringer's DIG Labeling and Detection kit b/c I'd
>like for our lab to switch over (me, at least) to non-radioactive probing.
>
>The instructions call for positively charged nylon membranes.  The one we
>have in lab is by Amersham and I imagine it is uncharged, since it didn't
>say anything on the box or instructions.
>
>I did my control labeled DNA filter and the spots are black.  This was on
>the Amersham filter.  SO is a positively charged filter truly needed?  I'd
>like to find out others' experiences before I go through the trouble of
>preparing a membrane with my experimental DNA
>
>TIA,
>
>Karen
Dear Karen 
I have tried the recommended Boe-Ma membrane(positively charged) and
the uncharged  Nytran membrane (Schleicher&Schuell) and I get less
specific staining and more background using the Boe-Ma.
Martin