Help with PCR adding sp6 sequence

william wu wwu at shriners.mcgill.ca
Mon Feb 3 16:04:52 EST 1997


Hi, I'm trying to add a sp6 sequence to my cDNA. All I have done is
added the sp6 sequence to the 5' end of my primer. I use the same
conditions as I do with the
regular primers. Guess what. The ones with the sp6 don't work while the
ones without it work beautifully. What gives??

-- 
William Wu	
PhD student
McGill University
Dept. of Experimental Surgery
Shriner's Hospital for Crippled Children
Joint Diseases Lab
Montreal,PQ.
Canada
http://www.geocities.com/yosemite/1156/index.html



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