Help with PCR adding sp6 sequence

william wu wwu at
Mon Feb 3 16:04:52 EST 1997

Hi, I'm trying to add a sp6 sequence to my cDNA. All I have done is
added the sp6 sequence to the 5' end of my primer. I use the same
conditions as I do with the
regular primers. Guess what. The ones with the sp6 don't work while the
ones without it work beautifully. What gives??

William Wu	
PhD student
McGill University
Dept. of Experimental Surgery
Shriner's Hospital for Crippled Children
Joint Diseases Lab

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