Primer Design

Mirna Perez mperez at FISIO.CINVESTAV.MX
Mon Feb 3 18:06:04 EST 1997


Hi!

I design an oligonucleotide for a PCR reaction, but I am not sure if its
lenght is enough (14bp) to performance a reaction. The oligo contains
12G/C and 2A/T, and its Tm is high (80"C). Is it lenght good enough to
do a PCR reaction?
			Thanks

			 Mirna



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