Q re: PCR from agarose plugs

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Mon Feb 3 09:29:23 EST 1997

WHat are the rules of thumb for re-eamplifying a band from a plug of
agarose?  I have had only variable success doing this, often getting a
smear or supernumerary bands instead of the target band alone. Is there a
standard protocol somewhere?

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