pH dependence of DNA hydrolysis

Shuiliang Shi sshi at cell-science.org
Mon Feb 3 09:29:21 EST 1997


I know that you can beat up your DNA to increase blotting efficiency for
Southern transfers by doing the "limited hydrolysis" with 0.1-0.2M HCl, and
my question leads from this common practice - at what pH does DNA start to
get torn up? And is the damage an actual nicking of the molecule, or is the
phosphodiester bond only broken by subsequent treatment with basic
solutions?  I can't find this info in any of the commonly used lab methods
books. thanks, dick lightfoot.




More information about the Methods mailing list