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subcloning large fragments

Frederic G. Barr Lab barrlab at MAIL.MED.UPENN.EDU
Tue Feb 4 16:20:45 EST 1997

When subcloning DNA fragments larger than 5 kb into pBluescript, I 
frequently recover DH5a mini-prep clones with various-sized deletions or 
no inserts. What reagents (bacterial strain, plasmid vector, restriction 
sites) should I be using so that I can subclone large (5-15 kb) DNA 
fragments that are intact?

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