Growth and transformation of XL1-Blue
Alexander N. Kukushkin
kan at mmcd.cyt.ras.spb.ru
Tue Feb 4 12:49:21 EST 1997
> I have XL1-blue cells and was wondering what the optimal conditions for
> growth and high-efficiency transformation? Can they be grown in LB
> medium containing 20mM magnesium sulphate and made competent using the
> rubidium chloride procedure described in Promega's "Protocols and
> Applications Guide"?
> Many thanks,
> Kevin Mulcahy.
> ps: If you need me to write out the transformation procedure then let me
> know and I'll post it to the list.
Recently I used XL1-blue cells for transformation. They were grown in
ordinary LB and transformed by usual CaCl2 procedure. The result was fine.
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