>> I have XL1-blue cells and was wondering what the optimal conditions for
> growth and high-efficiency transformation? Can they be grown in LB
> medium containing 20mM magnesium sulphate and made competent using the
> rubidium chloride procedure described in Promega's "Protocols and
> Applications Guide"?
>> Many thanks,
>> Kevin Mulcahy.
>> ps: If you need me to write out the transformation procedure then let me
> know and I'll post it to the list.
>Recently I used XL1-blue cells for transformation. They were grown in
ordinary LB and transformed by usual CaCl2 procedure. The result was fine.