: Mirna Perez (mperez at FISIO.CINVESTAV.MX) wrote:
: : Hi!
: : I design an oligonucleotide for a PCR reaction, but I am not sure if its
: : lenght is enough (14bp) to performance a reaction. The oligo contains
: : 12G/C and 2A/T, and its Tm is high (80"C). Is it lenght good enough to
: : do a PCR reaction?
: : Thanks
With that many G/Cs I would be very worried about the possibility of very
stable secondary structures forming within the oligo, which would prevent it
from annealing to your template.
Good luck, hope this helps,
: : Mirn
: i am afraid it may not be long enough tobe really specific,
: unless the DNA template is so pure that you are sure there is
: n othing else to amplifye! A quick way to test your primers
: is to screen Genbank with them. Also I think it is better
: to have a G/C content closer to 50%
: Good luck. Let me know the result of the search through databank.