GST-fusion protein extraction

Philippe Pognonec pognonec at unice.fr
Tue Feb 4 11:28:57 EST 1997


I am preparing my GST-fused proteins using the original procedure
(sonication in MTPBS). I have (more than) a feeling that this is far
from being a quantitative method of extraction.
Does anyone there know of a more efficient method for lyzing E. coli
while still retaining the ability to purify the fusion protein on
glutathione-agarose?

Thanx for your help!

Philippe Pognonec, Nice, France.



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