Growth and transformation of XL1-Blue

Fraser Dr N J njf16154 at ggr.co.uk
Tue Feb 4 08:38:00 EST 1997


"Kevin A. Mulcahy" <K.Mulcahy at sheffield.ac.uk> wrote:
>Hi,
>
>I have XL1-blue cells and was wondering what the optimal conditions for 
>growth and high-efficiency transformation? Can they be grown in LB 
>medium containing 20mM magnesium sulphate and made competent using the 
>rubidium chloride procedure described in Promega's "Protocols and 
>Applications Guide"?
>
>Many thanks,
>
>Kevin Mulcahy.

Yes - I've just done it with XL1-blue




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