Growth and transformation of XL1-Blue

Martin Offterdinger a8803349 at unet.univie.ac.at
Wed Feb 5 02:17:57 EST 1997


On 4 Feb 1997 17:49:21 -0000, "Alexander N. Kukushkin"
<kan at mmcd.cyt.ras.spb.ru> wrote:

>>   Hi,
>>
>>   I have XL1-blue cells and was wondering what the optimal conditions for
>>   growth and high-efficiency transformation? Can they be grown in LB
>>   medium containing 20mM magnesium sulphate and made competent using the
>>   rubidium chloride procedure described in Promega's "Protocols and
>>   Applications Guide"?
>>
>>   Many thanks,
>>
>>   Kevin Mulcahy.
>>
>>   ps: If you need me to write out the transformation procedure then let me
>>   know and I'll post it to the list.
>>
>Recently I used XL1-blue cells for transformation. They were grown in
>ordinary LB and transformed by usual CaCl2 procedure. The result was fine.
>Alex
I agree with Alex-I just used 100mM CaCl2 and it worked fine.



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