Karen Ferri wrote:
>> HI everyone!
>> I am currently trying Boehringer's DIG Labeling and Detection kit b/c I'd
> like for our lab to switch over (me, at least) to non-radioactive probing.
>> The instructions call for positively charged nylon membranes. The one we
> have in lab is by Amersham and I imagine it is uncharged, since it didn't
> say anything on the box or instructions.
>> I did my control labeled DNA filter and the spots are black. This was on
> the Amersham filter. SO is a positively charged filter truly needed? I'd
> like to find out others' experiences before I go through the trouble of
> preparing a membrane with my experimental DNA
We have been using Boehringer's DIG system since 1988 - and have used
all sorts of membrane, including theirs and Amersham's (chargd and
uncharged) and nitrocellulose. All work. Boehringer's probably works
best, but I don't personally think it worth the expense.
Ed Rybicki, PhD
Dept Microbiology | ed at molbiol.uct.ac.za
University of Cape Town | rybicki at uctvms.uct.ac.za
Private Bag, Rondebosch | phone: x27-21-650-3265
7700, South Africa | fax: x27-21-689 7573
WWW URL: http://www.uct.ac.za/microbiology/ed.html
"Out here on the perimeter, there are no stars..."