pH for RNA work
David L. Haviland, Ph.D.
dhavilan at IMM2.IMM.UTH.TMC.EDU
Wed Feb 5 15:07:42 EST 1997
At 10:35 2/5/97 -0800, Shi-Jye Chu wrote:
> I do not think so. It is only a crude guide. You must adjust with PH
>meter or paper.
True! I agree completely. Although good for a first approximation, the
Tris-Base/HCL combination like the mono- and di-phosphate buffers, can be
mixed per the Sigma or Isco tables. They are not absolute by any means but
often I find them as much as +/- 0.15 pH unit off. However, adjusting that
difference is much better than trying to pH Tris Base to 6.8 as some do...
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