IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

pH for RNA work

David L. Haviland, Ph.D. dhavilan at IMM2.IMM.UTH.TMC.EDU
Wed Feb 5 15:07:42 EST 1997

At 10:35 2/5/97 -0800, Shi-Jye Chu wrote:

>   I do not think so. It is only a crude guide. You must adjust with PH
>meter or paper.

True!   I agree completely.  Although good for a first approximation, the
Tris-Base/HCL combination like the mono- and di-phosphate buffers, can be
mixed per the Sigma or Isco tables.  They are not absolute by any means but
often I find them as much as +/- 0.15 pH unit off.  However, adjusting that
difference is much better than trying to pH Tris Base to 6.8 as some do...


More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net