Fragility of plasmids: size limit for shaking/vortexing?
kraev at bc.biol.ethz.ch
Wed Feb 5 14:04:15 EST 1997
In article <32F88C97.1431 at sheffield.ac.uk>, K.Mulcahy at sheffield.ac.uk wrote:
However, one of my plasmids is 10.5
> kb. Does anyone know if plasmids (pre-linearised) which are this large
> can be shaken vigorously without causing cleavage of the linear DNA
> fragment? If not, then what is the best way to extract them with
There is no upper limit as long as there is no standard for "vigorous
shaking by hand".
If you are worried about breakage, try Centriflex-AG cartridge from AGTC:
all you need is to centrifuge your sample through it , protein is trapped
in the cartridge.
Tel 001-301-990-2685, Cat No 10319. No affiliation.
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
e-mail kraev at bc.biol.ethz.ch
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