Methods for in vitro mutagenesis

Kellie Ann Lugenbeel kl690782 at bcm.tmc.edu
Wed Feb 5 11:49:52 EST 1997


I am looking for a protocol for doing in vitro mutagenesis. I only need
to alter one base in the coding region of a gene but the problem I am
having is that all the methods Ihave been referred to are dependent on
unique restriction sites that flank the region you want to mutate.  I am
having a hard time finding unique sites.  Does anyone knopw of a
protocol where maybe I wouln't need unique sites?

Thanks

Kellie Lugenbeel



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