How much DNA can be "crowded" into a solution?
e.hillery at ic.ac.uk
Wed Feb 5 08:06:19 EST 1997
>If I have a plasmid of molecular weight 10^6, what is the maximum
>concentration a solution can hold of this molecule? 1 Mol/L would
>indicate that 1000 kg would have to be solved in one L, which is
>clearly absurd. 10^-3 Mol/L would mean that 1 kg plasmid would have to
>solved in 1 L. Unrealistic? I don't know. Something in the range of
>10^-4 - 10^-6 Mol/L sounds realistic, but this is just a guess.
>Anybody know anything about this? Respond in E-mail and thread.
>PS I would also like to know the average molecular weight of DNA. What
>is the average molecular weight of a stretch of dsDNA of x
>I don't know if this'll be of any help, but 10mg/ml of any plasmid solution
is really pushing it. Also, the average Mw of a base pair is 660 D I think
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