Heparin in shift assays

doering_t at mpimg-berlin-dahlem.mpg.de doering_t at mpimg-berlin-dahlem.mpg.de
Wed Feb 5 07:05:11 EST 1997


Hi netters,

I'll need some help in protocolls for shift assays. In some protocols
for DNA shift assays the is an addition of heparin to the binding reaction.
Does anybody know which effect heparin on the nucleic acid-protein interaction/bindig 
will have. Tho only effect I heard is the blocking of (unspecific) ionic binding of the 
protein to the nucleic acid.  Is this true and if so how will 
heparin block ionic interaktions.
Hope somebody could help me. Thanks a lot!




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