E.coli for Electroporation

Helen McBride helen.mcbride at genetics.utah.edu
Wed Feb 5 17:43:50 EST 1997


In article <Pine.OSF.3.95.970125173430.2874A-100000 at saturn.mahidol.ac.th>
Panapat Uawithya, u3601087 at mahidol.ac.th writes:
>Hello  all,
> We are now using E.Coli JM109 for transformation by CaCl2. But it didn't
>produce good enough efficiency. Can it be use for electroporation or not?
>If it can't , can you suggest any E.coli strain that similar to this
>strain but can be use for electroporation.
>					Thank you in advance,
>					Panapat Uawithya


JM109 can be used for electroporation with standard protocols. Good luck.
Helen McBride
University of Utah 
Grad Student
Dept. of Onc. Sci.
helen.mcbride at genetics.utah.edu


"Where the telescope ends, the microscope begins, which of the two has
the grander view?" Victor Hugo



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