RNA extraction from little tissue.

Hiranya Roychowdhury hroychow at NMSU.EDU
Wed Feb 5 18:40:57 EST 1997


Hello netters,
        I am looking for some feedback on the use of conventional RNA extraction protocols versus the use of the commercially available columns (eg. Pharmacia, Qiagen etc.) when you are dealing with precious little samples. One of our grad students haven't had much luck with the Qia column the one time she tried. I will be shortly extracting some RNA from small amounts of *plant* tissues. Any pointer, experiences, tips etc. will be greatly appreciated. The RNA will be further enriched for polyA+ for, perhaps, cDNA construction. 

        Try and mail responses directly to me (ie. delete the Cc: <this_list>) so as not to bother others. I will repost a compilation of the responses to the net myself.

Hiranya.

----

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu



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