GST-fusion protein extraction

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Feb 5 16:01:36 EST 1997


Philippe Pognonec (pognonec at unice.fr) wrote:
> I am preparing my GST-fused proteins using the original procedure
> (sonication in MTPBS). I have (more than) a feeling that this is far
> from being a quantitative method of extraction.

You mean, you find GST-fusion protein in your pellet?

Inclusion bodies :-(

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



More information about the Methods mailing list