bright specks in EtBr stained gels - out, damn'd spot!
sam.michaelson at dsto.defence.gov.au
Thu Feb 6 11:56:37 EST 1997
Dear Netter Nutters
I've recently been having problems with very intense small bright spots
showing up on agarose gels stained with EtBr. I'm running 1.5% gels in
TBE, and staining the gels in 0.5 ug/ml EtBr after the run. I thought it
might be dust or crud getting into the mix; I'm using mol.biol. grade
agarose, and filtering the TBE carefully before use, although this hasn't
completely removed the problem. I'm trying to produce gels of a suitable
quality to be photographed for a poster. Has anyone else had this
problem, and if so, how did you tackle it?
bewildered biochemist now masquerading as molecular biologist
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