bright specks in EtBr stained gels - out, damn'd spot!

David Winterbourne sghk100 at sghms.ac.uk
Thu Feb 6 04:08:52 EST 1997


In article <32FA0D45.5A83 at dsto.defence.gov.au>, Sam Michaelson says...

>I've recently been having problems with very intense small bright spots 
>showing up on agarose gels stained with EtBr.  I'm running 1.5% gels in 
>TBE, and staining the gels in 0.5 ug/ml EtBr after the run.  I thought it 
>might be dust or crud getting into the mix; I'm using mol.biol. grade 
>agarose, and filtering the TBE carefully before use, although this hasn't 
>completely removed the problem.

I also used to filter the buffer until I realized the dust came mostly from 
the flask used to melt the agarose. Now I just rinse the flask in ultra pure 
water before preparing the gel (and I don't filter the buffer).

-- 
Dr. David Winterbourne
Department of Surgery
St George's Hospital Medical School, London SW17 0RE, England
Tel: 0181-725-5581   Fax: 0181-725-3594




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