220 RNA peak

Shelley Cole shelley at DARWIN.SFBR.ORG
Thu Feb 6 11:45:18 EST 1997


Wendy,

This might not be of much help, but are you sure that your spec is 
operating properly?

Shelley Cole
Staff Scientist
SW Foundation for Biomedical Research




On Tue, 4 Feb 1997, Wendy Stewart wrote:

>    Hi all;
>       We have been having an unsual RNA problem in our lab lately and
> since I have never seen this before, I don't quite know what to make of
> it.  We are harvesting total RNA from rat epithelial cell lines, using the
> traditional CsCl ultracentrifugation.  But instead of getting a lovely 260
> peak and an equally lovely ratio (which we still do get from time to
> time!) we are now picking up a very strong peak at 220; sometimes this is
> accompanied by a lesser peak at 260, sometimes it is there all by itself. 
> The yields are pretty good and they look O.K. (not fabulous) on
> formaldehyde gels.  My question is, what could be causing this absorbance
> at 220, why is the 260 there sometimes and sometimes not, and how does
> this impact on the quality of my total RNA?  We are not doing
> phenol/chloroform extractions, so this is not the problem.  Any thoughts
> anybody out there has would be greatly appreciated.
> 
> Thanks!
> 
> -- 
>                                    
> "Better living through chemistry"
> 
> 



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