220 RNA peak

Shelley Cole shelley at DARWIN.SFBR.ORG
Thu Feb 6 11:45:18 EST 1997


This might not be of much help, but are you sure that your spec is 
operating properly?

Shelley Cole
Staff Scientist
SW Foundation for Biomedical Research

On Tue, 4 Feb 1997, Wendy Stewart wrote:

>    Hi all;
>       We have been having an unsual RNA problem in our lab lately and
> since I have never seen this before, I don't quite know what to make of
> it.  We are harvesting total RNA from rat epithelial cell lines, using the
> traditional CsCl ultracentrifugation.  But instead of getting a lovely 260
> peak and an equally lovely ratio (which we still do get from time to
> time!) we are now picking up a very strong peak at 220; sometimes this is
> accompanied by a lesser peak at 260, sometimes it is there all by itself. 
> The yields are pretty good and they look O.K. (not fabulous) on
> formaldehyde gels.  My question is, what could be causing this absorbance
> at 220, why is the 260 there sometimes and sometimes not, and how does
> this impact on the quality of my total RNA?  We are not doing
> phenol/chloroform extractions, so this is not the problem.  Any thoughts
> anybody out there has would be greatly appreciated.
> Thanks!
> -- 
> "Better living through chemistry"

More information about the Methods mailing list