220 RNA peak
shelley at DARWIN.SFBR.ORG
Thu Feb 6 11:45:18 EST 1997
This might not be of much help, but are you sure that your spec is
SW Foundation for Biomedical Research
On Tue, 4 Feb 1997, Wendy Stewart wrote:
> Hi all;
> We have been having an unsual RNA problem in our lab lately and
> since I have never seen this before, I don't quite know what to make of
> it. We are harvesting total RNA from rat epithelial cell lines, using the
> traditional CsCl ultracentrifugation. But instead of getting a lovely 260
> peak and an equally lovely ratio (which we still do get from time to
> time!) we are now picking up a very strong peak at 220; sometimes this is
> accompanied by a lesser peak at 260, sometimes it is there all by itself.
> The yields are pretty good and they look O.K. (not fabulous) on
> formaldehyde gels. My question is, what could be causing this absorbance
> at 220, why is the 260 there sometimes and sometimes not, and how does
> this impact on the quality of my total RNA? We are not doing
> phenol/chloroform extractions, so this is not the problem. Any thoughts
> anybody out there has would be greatly appreciated.
> "Better living through chemistry"
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